No, it doesn't change the sound. But it may change how fast the gain increases per digit depending on the "taper" of the pot. An audio taper pot wired backwards would have a much more rapid increase in gain as the knob is turned than it would wired correctly. All the same settings will be there, but at different amounts of rotation. It won't behave like a mirror image unless you were using a linear taper pot to begin with. All the same tones will be there, and they will sound exactly the same.
Yes... Sorta. It depends on how much tone shaping you do in between stages also. RC networks also change the phase, though any one circuit won't change it much. But if you have alot of them the overall shift can be considerable. This is how a tube tremolo circuit works. A series of RC circuits flips the phase around 180* to create a positive feedback loop.
But overall your OK just counting stages. Better still, just listen to the amp normal and then wire the speaker in reverse and decide which you like better. The difference will seem very subtle. You won't even notice on very clean settings. But the harmonic order will change when overdriving the amp. Also, if you plan to run two amps at the same time and you find they are out of phase, you can try switching the speaker leads on one of them to see if you get a fuller tone. If you do, the amps were out of phase with respect to each other.
IME an amp that plays "foreward" will have better fundamental note sustain and an amp that plays "backward" will go to a sympathetic note instead.
Chuck


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